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apc conjugated mouse anti chicken cd3  (SouthernBiotech)


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    SouthernBiotech apc conjugated mouse anti chicken cd3
    Figure 1 Detection of ALV-J shedding, ALV-J viremia, ALV-J antibody and T lymphocyte percentage of B2 haplotype chickens after ALV-J infection. Six chickens were randomly selected for sampling every 7 days post-infection (dpi). A ALV-J shedding was monitored via detecting the p27 expression levels in cloacal swabs. S/P value below 0.2 indicated negative ALV-J shedding. B ALV-J viremia was monitored via virus isolation. An S/P value above 0.2 indicated positive ALV-J viremia. C The ALV-J antibody level in the serum was monitored using the commercial ALV-J antibody test kit. An S/P value above 0.6 was considered ALV-J antibody positive. Five days before infection (dbi) and each week after infection, PBMCs derived from five chickens of infected and control groups were isolated to detect the T lymphocyte percentage, including the percentage of the <t>CD4+CD3+T</t> cell (D), the <t>CD8α+CD3+T</t> cell (E), and the CD3+CD4+CD8α+T cell (F). Each sample collected 1 × 105 cells for flow cytometric analysis. The one-way test was used for statistical comparison among A, B and C. And the unpaired Student t test was used for statistical comparison among D, E and F. * P < 0.05, ** P < 0.01.
    Apc Conjugated Mouse Anti Chicken Cd3, supplied by SouthernBiotech, used in various techniques. Bioz Stars score: 94/100, based on 75 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/apc+conjugated+mouse+anti+chicken+cd3/Mouse+Anti-Chicken+IgM-APC/pm39696681-42-0-12
    Average 94 stars, based on 75 article reviews
    apc conjugated mouse anti chicken cd3 - by Bioz Stars, 2026-09
    94/100 stars

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    1) Product Images from "Revealing novel and conservative CD8 + T-cell epitopes with MHC B2 restriction on ALV-J."

    Article Title: Revealing novel and conservative CD8 + T-cell epitopes with MHC B2 restriction on ALV-J.

    Journal: Veterinary research

    doi: 10.1186/s13567-024-01426-3

    Figure 1 Detection of ALV-J shedding, ALV-J viremia, ALV-J antibody and T lymphocyte percentage of B2 haplotype chickens after ALV-J infection. Six chickens were randomly selected for sampling every 7 days post-infection (dpi). A ALV-J shedding was monitored via detecting the p27 expression levels in cloacal swabs. S/P value below 0.2 indicated negative ALV-J shedding. B ALV-J viremia was monitored via virus isolation. An S/P value above 0.2 indicated positive ALV-J viremia. C The ALV-J antibody level in the serum was monitored using the commercial ALV-J antibody test kit. An S/P value above 0.6 was considered ALV-J antibody positive. Five days before infection (dbi) and each week after infection, PBMCs derived from five chickens of infected and control groups were isolated to detect the T lymphocyte percentage, including the percentage of the CD4+CD3+T cell (D), the CD8α+CD3+T cell (E), and the CD3+CD4+CD8α+T cell (F). Each sample collected 1 × 105 cells for flow cytometric analysis. The one-way test was used for statistical comparison among A, B and C. And the unpaired Student t test was used for statistical comparison among D, E and F. * P < 0.05, ** P < 0.01.
    Figure Legend Snippet: Figure 1 Detection of ALV-J shedding, ALV-J viremia, ALV-J antibody and T lymphocyte percentage of B2 haplotype chickens after ALV-J infection. Six chickens were randomly selected for sampling every 7 days post-infection (dpi). A ALV-J shedding was monitored via detecting the p27 expression levels in cloacal swabs. S/P value below 0.2 indicated negative ALV-J shedding. B ALV-J viremia was monitored via virus isolation. An S/P value above 0.2 indicated positive ALV-J viremia. C The ALV-J antibody level in the serum was monitored using the commercial ALV-J antibody test kit. An S/P value above 0.6 was considered ALV-J antibody positive. Five days before infection (dbi) and each week after infection, PBMCs derived from five chickens of infected and control groups were isolated to detect the T lymphocyte percentage, including the percentage of the CD4+CD3+T cell (D), the CD8α+CD3+T cell (E), and the CD3+CD4+CD8α+T cell (F). Each sample collected 1 × 105 cells for flow cytometric analysis. The one-way test was used for statistical comparison among A, B and C. And the unpaired Student t test was used for statistical comparison among D, E and F. * P < 0.05, ** P < 0.01.

    Techniques Used: Infection, Sampling, Expressing, Virus, Isolation, Derivative Assay, Control, Comparison

    Related Articles

    Single Cell:

    Article Title: Systematic Identification of Host Immune Key Factors Influencing Viral Infection in PBL of ALV-J Infected SPF Chicken
    Article Snippet: .. 3 × 10 5 cells of PBL or tissue single-cell suspensions were simultaneously incubated with APC-conjugated mouse anti-chicken CD3 + , FITC-conjugated mouse anti-chicken CD4 + , and PE-conjugated mouse anti-chicken CD8α + monoclonal antibodies (SouthernBiotech, Birmingham, USA) in the dark at 4 °C for 30 min. After three washes with PBS, the labeled cells were analyzed by flow cytometer (CytoFLEX, Beckman Coulter, Brea, CA, USA) within 12 h. The data were analyzed by the software of FlowJo V10 (TreestarInc, Ashland, OR, USA). .. For the phenotype identification of the CD8 + T cell, FITC-conjugated mouse anti-chicken CD8β + and APC-conjugated mouse anti-chicken CD4 + monoclonal antibodies (SouthernBiotech, Birmingham, USA) were also used.

    Incubation:

    Article Title: Systematic Identification of Host Immune Key Factors Influencing Viral Infection in PBL of ALV-J Infected SPF Chicken
    Article Snippet: .. 3 × 10 5 cells of PBL or tissue single-cell suspensions were simultaneously incubated with APC-conjugated mouse anti-chicken CD3 + , FITC-conjugated mouse anti-chicken CD4 + , and PE-conjugated mouse anti-chicken CD8α + monoclonal antibodies (SouthernBiotech, Birmingham, USA) in the dark at 4 °C for 30 min. After three washes with PBS, the labeled cells were analyzed by flow cytometer (CytoFLEX, Beckman Coulter, Brea, CA, USA) within 12 h. The data were analyzed by the software of FlowJo V10 (TreestarInc, Ashland, OR, USA). .. For the phenotype identification of the CD8 + T cell, FITC-conjugated mouse anti-chicken CD8β + and APC-conjugated mouse anti-chicken CD4 + monoclonal antibodies (SouthernBiotech, Birmingham, USA) were also used.

    Article Title: Comparative analysis of key immune protection factors in H9N2 avian influenza viruses infected and immunized specific pathogen–free chicken
    Article Snippet: .. The 3 × 10 5 cells of PBL were simultaneously incubated with APC-conjugated mouse anti-chicken CD3 + , FITC-conjugated mouse anti-chicken CD4 + , and PE-conjugated mouse anti-chicken CD8α + monoclonal antibodies (SouthernBiotech, Birmingham, AL) in the dark at 4°C for 30 min. After 3 washes with PBS, the labeled cells were analyzed by flow cytometer (CytoFLEX; Beckman Coulter, Brea, CA) within 12 h. The data were analyzed by the software of FlowJo, V10 (TreestarInc, Ashland, OR). ..

    Article Title: Revealing novel and conservative T-cell epitopes with MHC B2 restriction on H9N2 Avian Influenza Virus (AIV).
    Article Snippet: This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record.. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article.. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.

    Bioprocessing:

    Article Title: Systematic Identification of Host Immune Key Factors Influencing Viral Infection in PBL of ALV-J Infected SPF Chicken
    Article Snippet: .. 3 × 10 5 cells of PBL or tissue single-cell suspensions were simultaneously incubated with APC-conjugated mouse anti-chicken CD3 + , FITC-conjugated mouse anti-chicken CD4 + , and PE-conjugated mouse anti-chicken CD8α + monoclonal antibodies (SouthernBiotech, Birmingham, USA) in the dark at 4 °C for 30 min. After three washes with PBS, the labeled cells were analyzed by flow cytometer (CytoFLEX, Beckman Coulter, Brea, CA, USA) within 12 h. The data were analyzed by the software of FlowJo V10 (TreestarInc, Ashland, OR, USA). .. For the phenotype identification of the CD8 + T cell, FITC-conjugated mouse anti-chicken CD8β + and APC-conjugated mouse anti-chicken CD4 + monoclonal antibodies (SouthernBiotech, Birmingham, USA) were also used.

    Article Title: Comparative analysis of key immune protection factors in H9N2 avian influenza viruses infected and immunized specific pathogen–free chicken
    Article Snippet: .. The 3 × 10 5 cells of PBL were simultaneously incubated with APC-conjugated mouse anti-chicken CD3 + , FITC-conjugated mouse anti-chicken CD4 + , and PE-conjugated mouse anti-chicken CD8α + monoclonal antibodies (SouthernBiotech, Birmingham, AL) in the dark at 4°C for 30 min. After 3 washes with PBS, the labeled cells were analyzed by flow cytometer (CytoFLEX; Beckman Coulter, Brea, CA) within 12 h. The data were analyzed by the software of FlowJo, V10 (TreestarInc, Ashland, OR). ..

    Article Title: Revealing novel and conservative T-cell epitopes with MHC B2 restriction on H9N2 Avian Influenza Virus (AIV).
    Article Snippet: This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record.. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article.. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.

    Labeling:

    Article Title: Systematic Identification of Host Immune Key Factors Influencing Viral Infection in PBL of ALV-J Infected SPF Chicken
    Article Snippet: .. 3 × 10 5 cells of PBL or tissue single-cell suspensions were simultaneously incubated with APC-conjugated mouse anti-chicken CD3 + , FITC-conjugated mouse anti-chicken CD4 + , and PE-conjugated mouse anti-chicken CD8α + monoclonal antibodies (SouthernBiotech, Birmingham, USA) in the dark at 4 °C for 30 min. After three washes with PBS, the labeled cells were analyzed by flow cytometer (CytoFLEX, Beckman Coulter, Brea, CA, USA) within 12 h. The data were analyzed by the software of FlowJo V10 (TreestarInc, Ashland, OR, USA). .. For the phenotype identification of the CD8 + T cell, FITC-conjugated mouse anti-chicken CD8β + and APC-conjugated mouse anti-chicken CD4 + monoclonal antibodies (SouthernBiotech, Birmingham, USA) were also used.

    Article Title: Comparative analysis of key immune protection factors in H9N2 avian influenza viruses infected and immunized specific pathogen–free chicken
    Article Snippet: .. The 3 × 10 5 cells of PBL were simultaneously incubated with APC-conjugated mouse anti-chicken CD3 + , FITC-conjugated mouse anti-chicken CD4 + , and PE-conjugated mouse anti-chicken CD8α + monoclonal antibodies (SouthernBiotech, Birmingham, AL) in the dark at 4°C for 30 min. After 3 washes with PBS, the labeled cells were analyzed by flow cytometer (CytoFLEX; Beckman Coulter, Brea, CA) within 12 h. The data were analyzed by the software of FlowJo, V10 (TreestarInc, Ashland, OR). ..

    Flow Cytometry:

    Article Title: Systematic Identification of Host Immune Key Factors Influencing Viral Infection in PBL of ALV-J Infected SPF Chicken
    Article Snippet: .. 3 × 10 5 cells of PBL or tissue single-cell suspensions were simultaneously incubated with APC-conjugated mouse anti-chicken CD3 + , FITC-conjugated mouse anti-chicken CD4 + , and PE-conjugated mouse anti-chicken CD8α + monoclonal antibodies (SouthernBiotech, Birmingham, USA) in the dark at 4 °C for 30 min. After three washes with PBS, the labeled cells were analyzed by flow cytometer (CytoFLEX, Beckman Coulter, Brea, CA, USA) within 12 h. The data were analyzed by the software of FlowJo V10 (TreestarInc, Ashland, OR, USA). .. For the phenotype identification of the CD8 + T cell, FITC-conjugated mouse anti-chicken CD8β + and APC-conjugated mouse anti-chicken CD4 + monoclonal antibodies (SouthernBiotech, Birmingham, USA) were also used.

    Article Title: Comparative analysis of key immune protection factors in H9N2 avian influenza viruses infected and immunized specific pathogen–free chicken
    Article Snippet: .. The 3 × 10 5 cells of PBL were simultaneously incubated with APC-conjugated mouse anti-chicken CD3 + , FITC-conjugated mouse anti-chicken CD4 + , and PE-conjugated mouse anti-chicken CD8α + monoclonal antibodies (SouthernBiotech, Birmingham, AL) in the dark at 4°C for 30 min. After 3 washes with PBS, the labeled cells were analyzed by flow cytometer (CytoFLEX; Beckman Coulter, Brea, CA) within 12 h. The data were analyzed by the software of FlowJo, V10 (TreestarInc, Ashland, OR). ..

    Article Title: Revealing novel and conservative T-cell epitopes with MHC B2 restriction on H9N2 Avian Influenza Virus (AIV).
    Article Snippet: This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record.. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article.. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.

    Software:

    Article Title: Systematic Identification of Host Immune Key Factors Influencing Viral Infection in PBL of ALV-J Infected SPF Chicken
    Article Snippet: .. 3 × 10 5 cells of PBL or tissue single-cell suspensions were simultaneously incubated with APC-conjugated mouse anti-chicken CD3 + , FITC-conjugated mouse anti-chicken CD4 + , and PE-conjugated mouse anti-chicken CD8α + monoclonal antibodies (SouthernBiotech, Birmingham, USA) in the dark at 4 °C for 30 min. After three washes with PBS, the labeled cells were analyzed by flow cytometer (CytoFLEX, Beckman Coulter, Brea, CA, USA) within 12 h. The data were analyzed by the software of FlowJo V10 (TreestarInc, Ashland, OR, USA). .. For the phenotype identification of the CD8 + T cell, FITC-conjugated mouse anti-chicken CD8β + and APC-conjugated mouse anti-chicken CD4 + monoclonal antibodies (SouthernBiotech, Birmingham, USA) were also used.

    Article Title: Comparative analysis of key immune protection factors in H9N2 avian influenza viruses infected and immunized specific pathogen–free chicken
    Article Snippet: .. The 3 × 10 5 cells of PBL were simultaneously incubated with APC-conjugated mouse anti-chicken CD3 + , FITC-conjugated mouse anti-chicken CD4 + , and PE-conjugated mouse anti-chicken CD8α + monoclonal antibodies (SouthernBiotech, Birmingham, AL) in the dark at 4°C for 30 min. After 3 washes with PBS, the labeled cells were analyzed by flow cytometer (CytoFLEX; Beckman Coulter, Brea, CA) within 12 h. The data were analyzed by the software of FlowJo, V10 (TreestarInc, Ashland, OR). ..



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    SouthernBiotech apc conjugated mouse anti chicken cd3
    Figure 1 Detection of ALV-J shedding, ALV-J viremia, ALV-J antibody and T lymphocyte percentage of B2 haplotype chickens after ALV-J infection. Six chickens were randomly selected for sampling every 7 days post-infection (dpi). A ALV-J shedding was monitored via detecting the p27 expression levels in cloacal swabs. S/P value below 0.2 indicated negative ALV-J shedding. B ALV-J viremia was monitored via virus isolation. An S/P value above 0.2 indicated positive ALV-J viremia. C The ALV-J antibody level in the serum was monitored using the commercial ALV-J antibody test kit. An S/P value above 0.6 was considered ALV-J antibody positive. Five days before infection (dbi) and each week after infection, PBMCs derived from five chickens of infected and control groups were isolated to detect the T lymphocyte percentage, including the percentage of the <t>CD4+CD3+T</t> cell (D), the <t>CD8α+CD3+T</t> cell (E), and the CD3+CD4+CD8α+T cell (F). Each sample collected 1 × 105 cells for flow cytometric analysis. The one-way test was used for statistical comparison among A, B and C. And the unpaired Student t test was used for statistical comparison among D, E and F. * P < 0.05, ** P < 0.01.
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    Image Search Results


    Figure 1 Detection of ALV-J shedding, ALV-J viremia, ALV-J antibody and T lymphocyte percentage of B2 haplotype chickens after ALV-J infection. Six chickens were randomly selected for sampling every 7 days post-infection (dpi). A ALV-J shedding was monitored via detecting the p27 expression levels in cloacal swabs. S/P value below 0.2 indicated negative ALV-J shedding. B ALV-J viremia was monitored via virus isolation. An S/P value above 0.2 indicated positive ALV-J viremia. C The ALV-J antibody level in the serum was monitored using the commercial ALV-J antibody test kit. An S/P value above 0.6 was considered ALV-J antibody positive. Five days before infection (dbi) and each week after infection, PBMCs derived from five chickens of infected and control groups were isolated to detect the T lymphocyte percentage, including the percentage of the CD4+CD3+T cell (D), the CD8α+CD3+T cell (E), and the CD3+CD4+CD8α+T cell (F). Each sample collected 1 × 105 cells for flow cytometric analysis. The one-way test was used for statistical comparison among A, B and C. And the unpaired Student t test was used for statistical comparison among D, E and F. * P < 0.05, ** P < 0.01.

    Journal: Veterinary research

    Article Title: Revealing novel and conservative CD8 + T-cell epitopes with MHC B2 restriction on ALV-J.

    doi: 10.1186/s13567-024-01426-3

    Figure Lengend Snippet: Figure 1 Detection of ALV-J shedding, ALV-J viremia, ALV-J antibody and T lymphocyte percentage of B2 haplotype chickens after ALV-J infection. Six chickens were randomly selected for sampling every 7 days post-infection (dpi). A ALV-J shedding was monitored via detecting the p27 expression levels in cloacal swabs. S/P value below 0.2 indicated negative ALV-J shedding. B ALV-J viremia was monitored via virus isolation. An S/P value above 0.2 indicated positive ALV-J viremia. C The ALV-J antibody level in the serum was monitored using the commercial ALV-J antibody test kit. An S/P value above 0.6 was considered ALV-J antibody positive. Five days before infection (dbi) and each week after infection, PBMCs derived from five chickens of infected and control groups were isolated to detect the T lymphocyte percentage, including the percentage of the CD4+CD3+T cell (D), the CD8α+CD3+T cell (E), and the CD3+CD4+CD8α+T cell (F). Each sample collected 1 × 105 cells for flow cytometric analysis. The one-way test was used for statistical comparison among A, B and C. And the unpaired Student t test was used for statistical comparison among D, E and F. * P < 0.05, ** P < 0.01.

    Article Snippet: APC-conjugated mouse anti-chicken CD3+, PE-conjugated mouse anti-chicken CD8α+, FITC-conjugated mouse anti-chicken CD4+, (SouthernBiotech, Birmingham, USA) were used in this study.

    Techniques: Infection, Sampling, Expressing, Virus, Isolation, Derivative Assay, Control, Comparison

    Detection of immune response in B2 haplotype chickens after H9N2 AIV infected. A , gating strategy of T lymphocyte from PBMCs after H9N2 AIV infection. B – D , four chickens from the infected and control groups were randomly selected for sampling to detect the percentage of CD3 + CD8α + T cells ( B ), the percentage of CD3 + CD4 + T cells ( C ), and the ratio of CD3 + CD4 + CD8α + T cells ( D ). 1.5 × 10 5 cells per sample were collected for flow cytometric analysis. Expressions of immune-related genes in PBMCs were detected by quantitative real-time polymerase chain reaction (qRT-PCR). In the H9N2 AIV-infected experiments, the total RNA of PBMC was extracted from chickens at 5 DPI. E – G , the transcriptional expressions of immune-related genes, including cytotoxicity-associated genes ( E ), innate immunity-related genes ( F ), and Th2 genes ( G ) were analyzed. Furthermore, data were collected from three biological samples in each group, and each sample performed in triplicate. The results were presented as means ± SEM, and the unpaired t test was used for statistical comparison of T lymphocyte percentage, and the paired t test was used for statistical comparison of expressions of immune-related genes. ∗p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001. AIV, avian influenza virus; DPI, days postinfection; IFN-γ, interferon-γ; PBMCs, peripheral blood mononuclear cells.

    Journal: The Journal of Biological Chemistry

    Article Title: Revealing novel and conservative T-cell epitopes with MHC B2 restriction on H9N2 avian influenza virus (AIV)

    doi: 10.1016/j.jbc.2024.107395

    Figure Lengend Snippet: Detection of immune response in B2 haplotype chickens after H9N2 AIV infected. A , gating strategy of T lymphocyte from PBMCs after H9N2 AIV infection. B – D , four chickens from the infected and control groups were randomly selected for sampling to detect the percentage of CD3 + CD8α + T cells ( B ), the percentage of CD3 + CD4 + T cells ( C ), and the ratio of CD3 + CD4 + CD8α + T cells ( D ). 1.5 × 10 5 cells per sample were collected for flow cytometric analysis. Expressions of immune-related genes in PBMCs were detected by quantitative real-time polymerase chain reaction (qRT-PCR). In the H9N2 AIV-infected experiments, the total RNA of PBMC was extracted from chickens at 5 DPI. E – G , the transcriptional expressions of immune-related genes, including cytotoxicity-associated genes ( E ), innate immunity-related genes ( F ), and Th2 genes ( G ) were analyzed. Furthermore, data were collected from three biological samples in each group, and each sample performed in triplicate. The results were presented as means ± SEM, and the unpaired t test was used for statistical comparison of T lymphocyte percentage, and the paired t test was used for statistical comparison of expressions of immune-related genes. ∗p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001. AIV, avian influenza virus; DPI, days postinfection; IFN-γ, interferon-γ; PBMCs, peripheral blood mononuclear cells.

    Article Snippet: 1 × 10 6 PBMCs were incubated with allophycocyanin-conjugated mouse anti-chicken CD3 + , FITC-conjugated mouse anti-chicken CD4 + , and PE-conjugated mouse anti-chicken CD8α + monoclonal antibodies (SouthernBiotech) in the dark at 4 °C for 30 min. After three washes with PBS, the ratios of CD3 + CD4 + T cells, CD3 + CD8α + T cells, and CD3 + CD4 + CD8α + T cells were analyzed by flow cytometry (CytoFLEX, Beckman Coulter).

    Techniques: Infection, Control, Sampling, Real-time Polymerase Chain Reaction, Quantitative RT-PCR, Comparison, Virus